Review



mouse α σ3 4f2  (Developmental Studies Hybridoma Bank)


Bioz Verified Symbol Developmental Studies Hybridoma Bank is a verified supplier
Bioz Manufacturer Symbol Developmental Studies Hybridoma Bank manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Developmental Studies Hybridoma Bank mouse α σ3 4f2
    Mouse α σ3 4f2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+%CE%B1+%CF%833+4f2/anti-Lim+1%2B2+%2F+LhxV5/pm40892861-353-27-32
    Average 95 stars, based on 88 article reviews
    mouse α σ3 4f2 - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Reovirus uses temporospatial compartmentalization to orchestrate core versus outercapsid assembly
    Article Snippet: .. Then, blocking solution was removed and cells were incubated with primary antibody solutions (block buffer containing: rabbit pAB against whole virus at 1:1000 (Biologics International Corp), rabbit pAB against core at 1:1000 (Biologics International Corp), mouse α-σ3 (4F2) at 1:250 (DSHB), mouse α-σ3 (10G10) at 1:250 (DSHB), mouse α-μ1 (10F6) at 1:1000 (DSHB), rabbit α-μNS at 1:1000 (Biologics International Corp), mouse α-σNS (2F5) at 1:500 (DSHB), mouse α-σ1 (G5) at 1:250 (DSHB), mouse α-σ3 (10C1 or 10G10) directly labelled with AlexaFluor 647 using the Apex AlexFluor 647 Ab-Labelling Kit (A10475, Invitrogen) at 1:100, mouse α-σ1 (G5) directly labelled with AlexaFluor 647 using the Apex AlexFluor 647 Ab-Labelling Kit, (Invitrogen) at 1:100, or mouse α-σNS (2A9) directly labelled with AlexaFluor 568 using the Apex AlexFluor 568 Ab-Labelling Kit, (A10494, Invitrogen) at 1:100 ) overnight at 4°C. ..

    Article Title: Reovirus uses temporospatial compartmentalization to orchestrate core versus outercapsid assembly
    Article Snippet: Following SDS-PAGE, proteins were transferred from the gel onto nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad). .. Membranes were incubated with block buffer (3% newborn calf serum (NCS)/TBS-T) for 1-2 hours at room temperature, followed by an incubation with a primary antibody solution (block buffer containing: rabbit pAB against whole virus at 1:10,000 (Biologics International Corp), rabbit pAB against core at 1:1000 (Biologics International Corp), mouse α-σ3 (4F2) at 1:1000 (Developmental Studies Hybridoma Bank (DSHB)), mouse α-μ1 (10F6) at 1:1000 (DSHB), rabbit α-μNS at 1:1000 (Biologics International Corp) and/or mouse α-σ1 (G5) at 1:500 (DSHB)) for either 1 hour at room temperature or overnight at 4°C followed by a secondary antibody solution (block buffer containing: goat α-rabbit AlexaFluor 488 or 647 at 1:1000, goat α-rabbit HRP 1:10,000, goat α-mouse AlexaFluor 488 or 647 at 1:1000, and/or goat α-mouse HRP 1:10,000) for 1 hour at room temperature. ..

    Article Title: Spatiotemporal coordination of reovirus peripheral core replication to perinuclear whole virus assembly
    Article Snippet: .. Then, blocking solution was removed and cells were incubated with primary antibody solutions (block buffer containing: rabbit polyclonal antibodies against core (α-Core) at 1:1000 (Biologics International Corp), mouse α-σ3 (4F2) at 1:250 (DSHB), rabbit polyclonal antibodies against μNS (α-μNS) at 1:1000 (Biologics International Corp), monoclonal mouse anti-tubulin (12G10, DSHB), monoclonal mouse anti-FLAG M2 (Sigma F1804), and/or polyclonal rabbit anti-V5 (Novus NB600–381)). overnight at 4 ̊C. ..

    Article Title: Spatiotemporal coordination of reovirus peripheral core replication to perinuclear whole virus assembly.
    Article Snippet: .. Then, blocking solution was removed and cells were incubated with primary antibody solutions (block buffer containing: rabbit polyclonal antibodies against core (α-Core) at 1:1000 (Biologics International Corp), mouse α-σ3 (4F2) at 1:250 (DSHB), rabbit polyclonal antibodies against μNS (α-μNS) at 1:1000 (Biologics International Corp), monoclonal mouse anti-tubulin (12G10, DSHB), monoclonal mouse anti-FLAG M2 (Sigma F1804), and/or polyclonal rabbit anti-V5 (Novus NB600–381)). overnight at 4 ̊C. ..

    Article Title: Reovirus uses temporospatial compartmentalization to orchestrate core versus outercapsid assembly
    Article Snippet: .. Then, blocking solution was removed and cells were incubated with primary antibody solutions (block buffer containing: rabbit pAB against whole virus at 1:1000 (Biologics International Corp), rabbit pAB against core at 1:1000 (Biologics International Corp), mouse α-σ3 (4F2) at 1:250 (DSHB), mouse α-σ3 (10G10) at 1:250 (DSHB), mouse α-μ1 (10F6) at 1:1000 (DSHB), rabbit α-μNS at 1:1000 (Biologics International Corp), mouse α-σNS (2F5) at 1:500 (DSHB), mouse α-σ1 (G5) at 1:250 (DSHB), mouse α-σ3 (10C1 or 10G10) directly labelled with AlexaFluor 647 using the Apex AlexFluor 647 Ab-Labelling Kit (A10475, Invitrogen) at 1:100, mouse α-σ1 (G5) directly labelled with AlexaFluor 647 using the Apex AlexFluor 647 Ab-Labelling Kit, (Invitrogen) at 1:100, or mouse α-σNS (2A9) directly labelled with AlexaFluor 568 using the Apex AlexFluor 568 Ab-Labelling Kit, (A10494, Invitrogen) at 1:100 overnight at 4°C. ..

    Article Title: Reovirus uses temporospatial compartmentalization to orchestrate core versus outercapsid assembly
    Article Snippet: Following SDS-PAGE, proteins were transferred from the gel onto nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad). .. Membranes were incubated with block buffer (3% newborn calf serum (NCS)/TBS-T) for 1–2 hours at room temperature, followed by an incubation with a primary antibody solution (block buffer containing: rabbit pAB against whole virus at 1:10,000 (Biologics International Corp), rabbit pAB against core at 1:1000 (Biologics International Corp), mouse α-σ3 (4F2) at 1:1000 (Developmental Studies Hybridoma Bank (DSHB)), mouse α-μ1 (10F6) at 1:1000 (DSHB), rabbit α-μNS at 1:1000 (Biologics International Corp) and/or mouse α-σ1 (G5) at 1:500 (DSHB)) for either 1 hour at room temperature or overnight at 4°C followed by a secondary antibody solution (block buffer containing: goat α-rabbit AlexaFluor 488 or 647 at 1:1000, goat α-rabbit HRP 1:10,000, goat α-mouse AlexaFluor 488 or 647 at 1:1000, and/or goat α-mouse HRP 1:10,000) for 1 hour at room temperature. ..

    Incubation:

    Article Title: Reovirus uses temporospatial compartmentalization to orchestrate core versus outercapsid assembly
    Article Snippet: .. Then, blocking solution was removed and cells were incubated with primary antibody solutions (block buffer containing: rabbit pAB against whole virus at 1:1000 (Biologics International Corp), rabbit pAB against core at 1:1000 (Biologics International Corp), mouse α-σ3 (4F2) at 1:250 (DSHB), mouse α-σ3 (10G10) at 1:250 (DSHB), mouse α-μ1 (10F6) at 1:1000 (DSHB), rabbit α-μNS at 1:1000 (Biologics International Corp), mouse α-σNS (2F5) at 1:500 (DSHB), mouse α-σ1 (G5) at 1:250 (DSHB), mouse α-σ3 (10C1 or 10G10) directly labelled with AlexaFluor 647 using the Apex AlexFluor 647 Ab-Labelling Kit (A10475, Invitrogen) at 1:100, mouse α-σ1 (G5) directly labelled with AlexaFluor 647 using the Apex AlexFluor 647 Ab-Labelling Kit, (Invitrogen) at 1:100, or mouse α-σNS (2A9) directly labelled with AlexaFluor 568 using the Apex AlexFluor 568 Ab-Labelling Kit, (A10494, Invitrogen) at 1:100 ) overnight at 4°C. ..

    Article Title: Reovirus uses temporospatial compartmentalization to orchestrate core versus outercapsid assembly
    Article Snippet: Following SDS-PAGE, proteins were transferred from the gel onto nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad). .. Membranes were incubated with block buffer (3% newborn calf serum (NCS)/TBS-T) for 1-2 hours at room temperature, followed by an incubation with a primary antibody solution (block buffer containing: rabbit pAB against whole virus at 1:10,000 (Biologics International Corp), rabbit pAB against core at 1:1000 (Biologics International Corp), mouse α-σ3 (4F2) at 1:1000 (Developmental Studies Hybridoma Bank (DSHB)), mouse α-μ1 (10F6) at 1:1000 (DSHB), rabbit α-μNS at 1:1000 (Biologics International Corp) and/or mouse α-σ1 (G5) at 1:500 (DSHB)) for either 1 hour at room temperature or overnight at 4°C followed by a secondary antibody solution (block buffer containing: goat α-rabbit AlexaFluor 488 or 647 at 1:1000, goat α-rabbit HRP 1:10,000, goat α-mouse AlexaFluor 488 or 647 at 1:1000, and/or goat α-mouse HRP 1:10,000) for 1 hour at room temperature. ..

    Article Title: Spatiotemporal coordination of reovirus peripheral core replication to perinuclear whole virus assembly
    Article Snippet: .. Then, blocking solution was removed and cells were incubated with primary antibody solutions (block buffer containing: rabbit polyclonal antibodies against core (α-Core) at 1:1000 (Biologics International Corp), mouse α-σ3 (4F2) at 1:250 (DSHB), rabbit polyclonal antibodies against μNS (α-μNS) at 1:1000 (Biologics International Corp), monoclonal mouse anti-tubulin (12G10, DSHB), monoclonal mouse anti-FLAG M2 (Sigma F1804), and/or polyclonal rabbit anti-V5 (Novus NB600–381)). overnight at 4 ̊C. ..

    Article Title: Spatiotemporal coordination of reovirus peripheral core replication to perinuclear whole virus assembly.
    Article Snippet: .. Then, blocking solution was removed and cells were incubated with primary antibody solutions (block buffer containing: rabbit polyclonal antibodies against core (α-Core) at 1:1000 (Biologics International Corp), mouse α-σ3 (4F2) at 1:250 (DSHB), rabbit polyclonal antibodies against μNS (α-μNS) at 1:1000 (Biologics International Corp), monoclonal mouse anti-tubulin (12G10, DSHB), monoclonal mouse anti-FLAG M2 (Sigma F1804), and/or polyclonal rabbit anti-V5 (Novus NB600–381)). overnight at 4 ̊C. ..

    Article Title: Reovirus uses temporospatial compartmentalization to orchestrate core versus outercapsid assembly
    Article Snippet: .. Then, blocking solution was removed and cells were incubated with primary antibody solutions (block buffer containing: rabbit pAB against whole virus at 1:1000 (Biologics International Corp), rabbit pAB against core at 1:1000 (Biologics International Corp), mouse α-σ3 (4F2) at 1:250 (DSHB), mouse α-σ3 (10G10) at 1:250 (DSHB), mouse α-μ1 (10F6) at 1:1000 (DSHB), rabbit α-μNS at 1:1000 (Biologics International Corp), mouse α-σNS (2F5) at 1:500 (DSHB), mouse α-σ1 (G5) at 1:250 (DSHB), mouse α-σ3 (10C1 or 10G10) directly labelled with AlexaFluor 647 using the Apex AlexFluor 647 Ab-Labelling Kit (A10475, Invitrogen) at 1:100, mouse α-σ1 (G5) directly labelled with AlexaFluor 647 using the Apex AlexFluor 647 Ab-Labelling Kit, (Invitrogen) at 1:100, or mouse α-σNS (2A9) directly labelled with AlexaFluor 568 using the Apex AlexFluor 568 Ab-Labelling Kit, (A10494, Invitrogen) at 1:100 overnight at 4°C. ..

    Article Title: Reovirus uses temporospatial compartmentalization to orchestrate core versus outercapsid assembly
    Article Snippet: Following SDS-PAGE, proteins were transferred from the gel onto nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad). .. Membranes were incubated with block buffer (3% newborn calf serum (NCS)/TBS-T) for 1–2 hours at room temperature, followed by an incubation with a primary antibody solution (block buffer containing: rabbit pAB against whole virus at 1:10,000 (Biologics International Corp), rabbit pAB against core at 1:1000 (Biologics International Corp), mouse α-σ3 (4F2) at 1:1000 (Developmental Studies Hybridoma Bank (DSHB)), mouse α-μ1 (10F6) at 1:1000 (DSHB), rabbit α-μNS at 1:1000 (Biologics International Corp) and/or mouse α-σ1 (G5) at 1:500 (DSHB)) for either 1 hour at room temperature or overnight at 4°C followed by a secondary antibody solution (block buffer containing: goat α-rabbit AlexaFluor 488 or 647 at 1:1000, goat α-rabbit HRP 1:10,000, goat α-mouse AlexaFluor 488 or 647 at 1:1000, and/or goat α-mouse HRP 1:10,000) for 1 hour at room temperature. ..

    Virus:

    Article Title: Reovirus uses temporospatial compartmentalization to orchestrate core versus outercapsid assembly
    Article Snippet: .. Then, blocking solution was removed and cells were incubated with primary antibody solutions (block buffer containing: rabbit pAB against whole virus at 1:1000 (Biologics International Corp), rabbit pAB against core at 1:1000 (Biologics International Corp), mouse α-σ3 (4F2) at 1:250 (DSHB), mouse α-σ3 (10G10) at 1:250 (DSHB), mouse α-μ1 (10F6) at 1:1000 (DSHB), rabbit α-μNS at 1:1000 (Biologics International Corp), mouse α-σNS (2F5) at 1:500 (DSHB), mouse α-σ1 (G5) at 1:250 (DSHB), mouse α-σ3 (10C1 or 10G10) directly labelled with AlexaFluor 647 using the Apex AlexFluor 647 Ab-Labelling Kit (A10475, Invitrogen) at 1:100, mouse α-σ1 (G5) directly labelled with AlexaFluor 647 using the Apex AlexFluor 647 Ab-Labelling Kit, (Invitrogen) at 1:100, or mouse α-σNS (2A9) directly labelled with AlexaFluor 568 using the Apex AlexFluor 568 Ab-Labelling Kit, (A10494, Invitrogen) at 1:100 ) overnight at 4°C. ..

    Article Title: Reovirus uses temporospatial compartmentalization to orchestrate core versus outercapsid assembly
    Article Snippet: Following SDS-PAGE, proteins were transferred from the gel onto nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad). .. Membranes were incubated with block buffer (3% newborn calf serum (NCS)/TBS-T) for 1-2 hours at room temperature, followed by an incubation with a primary antibody solution (block buffer containing: rabbit pAB against whole virus at 1:10,000 (Biologics International Corp), rabbit pAB against core at 1:1000 (Biologics International Corp), mouse α-σ3 (4F2) at 1:1000 (Developmental Studies Hybridoma Bank (DSHB)), mouse α-μ1 (10F6) at 1:1000 (DSHB), rabbit α-μNS at 1:1000 (Biologics International Corp) and/or mouse α-σ1 (G5) at 1:500 (DSHB)) for either 1 hour at room temperature or overnight at 4°C followed by a secondary antibody solution (block buffer containing: goat α-rabbit AlexaFluor 488 or 647 at 1:1000, goat α-rabbit HRP 1:10,000, goat α-mouse AlexaFluor 488 or 647 at 1:1000, and/or goat α-mouse HRP 1:10,000) for 1 hour at room temperature. ..

    Article Title: Reovirus uses temporospatial compartmentalization to orchestrate core versus outercapsid assembly
    Article Snippet: .. Then, blocking solution was removed and cells were incubated with primary antibody solutions (block buffer containing: rabbit pAB against whole virus at 1:1000 (Biologics International Corp), rabbit pAB against core at 1:1000 (Biologics International Corp), mouse α-σ3 (4F2) at 1:250 (DSHB), mouse α-σ3 (10G10) at 1:250 (DSHB), mouse α-μ1 (10F6) at 1:1000 (DSHB), rabbit α-μNS at 1:1000 (Biologics International Corp), mouse α-σNS (2F5) at 1:500 (DSHB), mouse α-σ1 (G5) at 1:250 (DSHB), mouse α-σ3 (10C1 or 10G10) directly labelled with AlexaFluor 647 using the Apex AlexFluor 647 Ab-Labelling Kit (A10475, Invitrogen) at 1:100, mouse α-σ1 (G5) directly labelled with AlexaFluor 647 using the Apex AlexFluor 647 Ab-Labelling Kit, (Invitrogen) at 1:100, or mouse α-σNS (2A9) directly labelled with AlexaFluor 568 using the Apex AlexFluor 568 Ab-Labelling Kit, (A10494, Invitrogen) at 1:100 overnight at 4°C. ..

    Article Title: Reovirus uses temporospatial compartmentalization to orchestrate core versus outercapsid assembly
    Article Snippet: Following SDS-PAGE, proteins were transferred from the gel onto nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad). .. Membranes were incubated with block buffer (3% newborn calf serum (NCS)/TBS-T) for 1–2 hours at room temperature, followed by an incubation with a primary antibody solution (block buffer containing: rabbit pAB against whole virus at 1:10,000 (Biologics International Corp), rabbit pAB against core at 1:1000 (Biologics International Corp), mouse α-σ3 (4F2) at 1:1000 (Developmental Studies Hybridoma Bank (DSHB)), mouse α-μ1 (10F6) at 1:1000 (DSHB), rabbit α-μNS at 1:1000 (Biologics International Corp) and/or mouse α-σ1 (G5) at 1:500 (DSHB)) for either 1 hour at room temperature or overnight at 4°C followed by a secondary antibody solution (block buffer containing: goat α-rabbit AlexaFluor 488 or 647 at 1:1000, goat α-rabbit HRP 1:10,000, goat α-mouse AlexaFluor 488 or 647 at 1:1000, and/or goat α-mouse HRP 1:10,000) for 1 hour at room temperature. ..



    Similar Products

    95
    Developmental Studies Hybridoma Bank mouse α σ3 4f2
    Mouse α σ3 4f2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+%CE%B1+%CF%833+4f2/anti-Lim+1%2B2+%2F+LhxV5/pm40892861-353-27-32
    Average 95 stars, based on 1 article reviews
    mouse α σ3 4f2 - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    Image Search Results